Radioimmunoassay Technique for Measuring Cortisol in Milk
نویسندگان
چکیده
منابع مشابه
[Radioimmunoassay of plasma cortisol].
A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated corti...
متن کاملAdaptation and validation of a radioimmunoassay kit for measuring plasma cortisol in turbot.
Levels of cortisol in fish blood provide quantitative information on the degree of stress induced by a variety of stressors. It is also useful in describing the social status of individual fish within groups. The commercial production of radioimmunoassay (RIA) kits, such as the DPC Coat-A-Count radioimmunoassay kit, has considerably reduced the effort required for cortisol measurement. These ki...
متن کاملParallel radioimmunoassay for plasma cortisol and 11-deoxycortisol.
We describe a direct, rapid, and specific procedure for the parallel radioimmunoassay for cortisol and 11-deoxycortisol in plasma. The plasma sample is used directly, after heat inactivation of the natural cortisol-binding protein. The radioimmunoassay utilizes antibodies generated in rabbits by steroids congugated at their 3-oxo position to thyroglobulin. Ammonium sulfate is used to separate b...
متن کاملA specific, non-chromatographic radioimmunoassay for human plasma cortisol.
A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mole) and capacity (2.3 X 10(-6) moles/L undiluted serum) for cortisol. The mini...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1980
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(80)82956-0